HPRP-A1-iRGD

General Information


DCTPep ID  DCTPep00859

Peptide Name   HPRP-A1-iRGD

Sequence  FKKLKKLFSKLWNWKCRGDKGPDC

Sequence Length  24

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A549 Lung adenocarcinoma IC50=14.3 µM MTT assay 24h 1
MCF-7 Invasive breast carcinoma of no special type IC50=14.8 µM MTT assay 24h 1
NCI-H460 Lung large cell carcinoma IC50=15.2 µM MTT assay 24h 1
BGC-823 Human papillomavirus-related endocervical adenocarcinoma IC50=18.5 µM MTT assay 24h 1
MDA-MB-231 Breast adenocarcinoma IC50=22.5 µM MTT assay 24h 1

Hemolytic Activity  Human red blood cells: MHC=125 μM, MHC: the minimum hemolytic concentration is the concentration of peptide that results in 10% hemolysis of human red blood cells (hRBCs).

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50=26.5 μM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00859

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C136H213N37O31S2

Absent amino acids  AEHIMQTVY

Theoretical pI  9.93

Acidic residues  2

Basic residues  8

Polar residues  6

Molecular weight (Average)  2926.54

Molecular weight (Monoisotopic)  2924.57

Common amino acids  K

Net charge  6

Instability index (II)  -13.9

Aliphatic index  48.75

Grand average of hydropathicity (GRAVY)  -1.054

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 11125
  Abs 0.1% (=1 g/l) 3.801, assuming all pairs of Cys residues form cystines
  Ext. coefficient 11000
  Abs 0.1% (=1 g/l) 3.759, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 30592418

Title  Targeted Modification of the Cationic Anticancer Peptide HPRP-A1 with iRGD To Improve Specificity, Penetration, and Tumor-Tissue Accumulation

Doi 10.1021/acs.molpharmaceut.8b00854

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.