This work is licensed under a Creative Commons Attribution 4.0 International License.
HPRP-A1-iRGD
General Information
DCTPep ID DCTPep00859
Peptide Name HPRP-A1-iRGD
Sequence FKKLKKLFSKLWNWKCRGDKGPDC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | IC50=14.3 µM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=14.8 µM | MTT assay | 24h | 1 |
| NCI-H460 | Lung large cell carcinoma | IC50=15.2 µM | MTT assay | 24h | 1 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | IC50=18.5 µM | MTT assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | IC50=22.5 µM | MTT assay | 24h | 1 |
Hemolytic Activity Human red blood cells: MHC=125 μM, MHC: the minimum hemolytic concentration is the concentration of peptide that results in 10% hemolysis of human red blood cells (hRBCs).
Normal (non-cancerous) Cytotoxicity HUVEC: IC50=26.5 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00859
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C136H213N37O31S2
Absent amino acids AEHIMQTVY
Theoretical pI 9.93
Acidic residues 2
Basic residues 8
Polar residues 6
Molecular weight (Average) 2926.54
Molecular weight (Monoisotopic) 2924.57
Common amino acids K
Net charge 6
Instability index (II) -13.9
Aliphatic index 48.75
Grand average of hydropathicity (GRAVY) -1.054
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11125
Abs 0.1% (=1 g/l) 3.801, assuming all pairs of Cys residues form cystines
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 3.759, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30592418
Title Targeted Modification of the Cationic Anticancer Peptide HPRP-A1 with iRGD To Improve Specificity, Penetration, and Tumor-Tissue Accumulation
Doi 10.1021/acs.molpharmaceut.8b00854
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.