This work is licensed under a Creative Commons Attribution 4.0 International License.
ChMAP-28
General Information
DCTPep ID DCTPep00863
Peptide Name ChMAP-28
Sequence GRFKRFRKKLKRLWHKVGPFVGPILHY
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin Goat Antimicrobial
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=3.39±0.15 μM | MTT assay | 48h | 1 |
| B16-F1 | Mouse melanoma | IC50=4.82±1.01 μM | MTT assay | 48h | 1 |
| SK-BR-3 | Breast adenocarcinoma | IC50=5.63±1.05 μM | MTT assay | 48h | 1 |
| A431 | Skin squamous cell carcinoma | IC50=6.49±0.09 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: ChMAP-28 has a low hemolytic activity (hemolysis less than 10%) at a concentration range up to 10 μM.
Normal (non-cancerous) Cytotoxicity HEK293T: IC50=5.09±0.40 μM; HEF: IC50=8.95±2.68 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00863
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C163H255N49O29
Absent amino acids ACDEMNQST
Theoretical pI 12.02
Acidic residues 0
Basic residues 11
Polar residues 4
Molecular weight (Average) 3365.13
Molecular weight (Monoisotopic) 3363
Common amino acids K
Net charge 11
Instability index (II) 69.34
Aliphatic index 79.26
Grand average of hydropathicity (GRAVY) -0.659
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.077
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30622471
Title Anticancer Activity of the Goat Antimicrobial Peptide ChMAP-28
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.