NoD173

General Information


DCTPep ID  DCTPep00866

Peptide Name   NoD173

Sequence  RQCKAESNTFTGICIAKPPCRQACIREKFTDGHCSKVLRRCLCTKRC

Sequence Length  47

UniProt ID  Not available

PubChem CID  Not available

Origin  Nicotiana occidentalis

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=1.35±0.06 μM MTT assay 48h 1
MM170 Melanoma IC50=1.75±0.06 μM MTT assay 48h 1
PC-3 Prostate carcinoma IC50=6.31±0.099 μM MTT assay 48h 1

Hemolytic Activity  Human red blood cells: with ∼12% lysis at the very high concentration (100 µM)

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50=12.31±0.08 μM; AHDF: IC50=10.17±0.29 μM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00866

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C222H377N75O63S8

Absent amino acids  MWY

Theoretical pI  9.45

Acidic residues  3

Basic residues  12

Polar residues  17

Molecular weight (Average)  5361.38

Molecular weight (Monoisotopic)  5357.64

Common amino acids  C

Net charge  9

Instability index (II)  60.37

Aliphatic index  54.04

Grand average of hydropathicity (GRAVY)  -0.485

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 500
  Abs 0.1% (=1 g/l) 0.093, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 30794440

Title  Structural and functional characterization of the membrane-permeabilizing activity of Nicotiana occidentalis defensin NoD173 and protein engineering to enhance oncolysis

Doi 10.1096/fj.201802540R

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.