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KT2
General Information
DCTPep ID DCTPep00869
Peptide Name KT2
Sequence NGVQPKYKWWKWWKKWW
Sequence Length 17
UniProt ID Not available
PubChem CID Not available
Origin AMP
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A431 | Skin squamous cell carcinoma | 34% Cytotoxicity=10 µM | MTT assay | 24h | 1 |
| HCT 116 | Colon carcinoma | IC50=50 µM | MTT assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Not active up to 10 µM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism KT2 interacts with the plasma membrane and is then internalized into cells. Moreover, the KT2 peptide increases membrane permeability and induces cytotoxicity against cancer cells through DNA condensation, the increase of apoptotic factor protein levels, and autophagy suppression.
Structure Information
PDB ID Not available
Predicted Structure DCTPep00869
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Cys2<--->Gly11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C126H164N30O21
Absent amino acids ACDEFHILMRST
Theoretical pI 10.30
Acidic residues 0
Basic residues 5
Polar residues 3
Molecular weight (Average) 2434.88
Molecular weight (Monoisotopic) 2433.27
Common amino acids W
Net charge 5
Instability index (II) 11.9
Aliphatic index 17.06
Grand average of hydropathicity (GRAVY) -1.824
Half Life
1.4 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 34490
Abs 0.1% (=1 g/l) 14.165
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31073999
Title The cationic cell-penetrating KT2 peptide promotes cell membrane defects and apoptosis with autophagy inhibition in human HCT 116 colon cancer cells
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.