Peptide Libraray

Mastoparan-L [I1K,N2I,A5R,L9K,A10I]

General Information

DCTPep ID DCTPep00874

Peptide Name Mastoparan-L [I1K,N2I,A5R,L9K,A10I]

Sequence KILKRLAAKIKKIL

Sequence Length 14

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

Cancer therapy related peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HT-29	Colon adenocarcinoma	IC50>100 µM	MTT assay	2h	1
MCF-7	Invasive breast carcinoma of no special type	IC50>100 µM	MTT assay	2h	1
PC-3	Prostate carcinoma	IC50>100 µM	MTT assay	2h	1

Hemolytic Activity Human erythrocytes: 3% Hemolysis=100 µM

Normal (non-cancerous) Cytotoxicity 3T3-L1: 50% Cell death>100 µM ; HUVEC: 50% Cell death>100 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00874

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C78H150N22O15

Absent amino acids CDEFGHMNPQSTVWY

Theoretical pI 11.39

Acidic residues 0

Basic residues 6

Polar residues 0

Molecular weight (Average) 1636.19

Molecular weight (Monoisotopic) 1635.17

Common amino acids K

Net charge 6

Instability index (II) 29.76

Aliphatic index 181.43

Grand average of hydropathicity (GRAVY) 0.321

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 31411881

Title Computer-Aided Design of Mastoparan-like Peptides Enables the Generation of Nontoxic Variants With Extended Antibacterial Properties

Doi 10.1021/acs.jmedchem.9b00915

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available