This work is licensed under a Creative Commons Attribution 4.0 International License.
[G18K]cTI
General Information
DCTPep ID DCTPep00918
Peptide Name [G18K]cTI
Sequence KWCFRVCYRGICYRRCRK
Sequence Length 18
PubChem CID Not available
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MM96L | Melanoma | CC50=1.0±0.1 µM | Resazurin assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=1.7±0.1 µM | Resazurin assay | 24h | 1 |
| WM164 | Cutaneous melanoma | CC50=2.7±0.2 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | CC50=3.2±0.1 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=6.7±1.1 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50=6.9±0.3 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50>64 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
Predicted Structure DCTPep00918
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C105H166N36O21S4
Absent amino acids ADEHLMNPQST
Theoretical pI 10.10
Acidic residues 0
Basic residues 7
Polar residues 7
Molecular weight (Average) 2396.94
Molecular weight (Monoisotopic) 2395.19
Common amino acids R
Net charge 7
Instability index (II) 13.04
Aliphatic index 37.78
Grand average of hydropathicity (GRAVY) -0.706
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 3.642, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.538, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.