Peptide Libraray

EeCentrocin 1 Chain A (1-12)[D8A, R11A, N12K]

General Information

DCTPep ID DCTPep00932

Peptide Name EeCentrocin 1 Chain A (1-12)[D8A, R11A, N12K]

Sequence GWWRRTVAKVAK

Sequence Length 12

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

Cancer therapy related peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
A2058	Amelanotic melanoma	IC50>200µM	MTS assay		1

Hemolytic Activity Human erythrocytes:0% Hemolysis=25-200 µM

Normal (non-cancerous) Cytotoxicity MRC-5: IC50>200 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C68H108N22O14

Absent amino acids CDEFHILMNPQSY

Theoretical pI 12.02

Acidic residues 0

Basic residues 4

Polar residues 2

Molecular weight (Average) 1457.74

Molecular weight (Monoisotopic) 1456.84

Common amino acids AKRVW

Net charge 4

Instability index (II) 60.11

Aliphatic index 65.00

Grand average of hydropathicity (GRAVY) -0.642

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 7.546

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 31802582

Title Structure-activity relationship studies of shortened analogues of the antimicrobial peptide EeCentrocin 1 from the sea urchin Echinus esculentus

Doi 10.1002/psc.3233

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available