Peptide Libraray

Indolicidin-Arg

General Information

DCTPep ID DCTPep00944

Peptide Name Indolicidin-Arg

Sequence ILPWKWPWWPWRR

Sequence Length 13

UniProt ID Not available

PubChem CID Not available

Origin porcine cathelicidin peptide

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
Jurkat	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=13.7 µM	MTT assay	24h	1
BMKC	Skin carcinoma	LD50=200 µg/ml	MTT assay	24 h	Patent
NCI-H1299	Lung large cell carcinoma	LD50=270 µg/ml	MTT assay	24 h	Patent
PC-3	Prostate carcinoma	LD50=290 µg/ml	MTT assay	24 h	Patent
HeLa	Human papillomavirus-related endocervical adenocarcinoma	LD50=345 µg/ml	MTT assay	24 h	Patent
SW480	Colon adenocarcinoma	LD50=345 µg/ml	MTT assay	24 h	Patent
MCF-7	Invasive breast carcinoma of no special type	LD50=64 µg/ml	MTT assay	24 h	Patent

Hemolytic Activity RBC: IC50 ≥ 65 µM

Normal (non-cancerous) Cytotoxicity PBMC: IC50=26.8 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00944

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C100H131N25O14

Absent amino acids ACDEFGHMNQSTVY

Theoretical pI 12.01

Acidic residues 0

Basic residues 3

Polar residues 0

Molecular weight (Average) 1907.3

Molecular weight (Monoisotopic) 1906.03

Common amino acids W

Net charge 3

Instability index (II) 76.28

Aliphatic index 60.00

Grand average of hydropathicity (GRAVY) -1.069

Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 14.418

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 32126228

Title Selective anticancer activity of synthetic peptides derived from the host defence peptide tritrpticin

Doi 10.1016/j.bbamem.2020.183228

Year 2020

Patent

Patent ID US2005/0209157A1

Patent Title Short bioactive peptides and methods for their use

Other Iinformation Patent Application; Family: 1s / 24ex; Family Jurisdictions: US; Legal Status: Discontinued; Application No: 13618605; Filed: May 24, 2005; Published: Sep 22, 2005; Earliest Priority: Mar 28, 2001

Other Published ID Not available