Hyen E

General Information


DCTPep ID  DCTPep00994

Peptide Name   Hyen E

Sequence  GVPCGESCVYIPCFTGIINCSCRDKVCYNN

Sequence Length  30

UniProt ID  C0HLN9 

PubChem CID  Not available

Origin  medicinal herb Hybanthus enneaspermus

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma CC50=2.64±0.09 µM Resazurin assay 24h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia CC50=2.85±0.09 µM Resazurin assay 24h 1
MCF-7 Invasive breast carcinoma of no special type CC50=3.73±0.25 µM Resazurin assay 24h 1

Hemolytic Activity  Human red blood cells (hRBCs): HC50>50 µM

Normal (non-cancerous) Cytotoxicity  HUVEC: CC50=2.06±0.08 µM , testing time is 24h

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00994

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys4<--->Cys20; Cys8<--->Cys22; Cys13<--->Cys27

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C137H213N37O43S6

Absent amino acids  AHLMQW

Theoretical pI  6.03

Acidic residues  2

Basic residues  2

Polar residues  17

Molecular weight (Average)  3258.78

Molecular weight (Monoisotopic)  3256.39

Common amino acids  C

Net charge  0

Instability index (II)  18.43

Aliphatic index  68.00

Grand average of hydropathicity (GRAVY)  0.290

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 3355
  Abs 0.1% (=1 g/l) 1.030, assuming all pairs of Cys residues form cystines
  Ext. coefficient 2980
  Abs 0.1% (=1 g/l) 0.914, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 32414842

Title  Discovery and mechanistic studies of cytotoxic cyclotides from the medicinal herb Hybanthus enneaspermus

Doi 10.1074/jbc.RA120.012627

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.