This work is licensed under a Creative Commons Attribution 4.0 International License.
Dermaseptin-PP
General Information
DCTPep ID DCTPep00997
Peptide Name Dermaseptin-PP
Sequence ALWKDMLKGIGKLAGKAALGAVKTLV
Sequence Length 26
UniProt ID Not available
PubChem CID Not available
Origin Phyllomedusa palliata Skin Secretions
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| NCI-H157 | Lung squamous cell carcinoma | IC50=1.55 μM | MTT assay | 24h | 1 |
| U-251MG | Astrocytoma | IC50=2.47 μM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=2.92 μM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=4.15 μM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: Dermaseptin-PP exhibited only a moderate hemolytic effect (<20% at 16 μM with a calculated HC50 of 38.77 μM)
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00997
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C123H214N32O30S1
Absent amino acids CEFHNPQRSY
Theoretical pI 10.18
Acidic residues 1
Basic residues 5
Polar residues 5
Molecular weight (Average) 2653.31
Molecular weight (Monoisotopic) 2651.59
Common amino acids AKL
Net charge 4
Instability index (II) -5.24
Aliphatic index 131.54
Grand average of hydropathicity (GRAVY) 0.638
Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.073
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 32582642
Title Novel Frog Skin-Derived Peptide Dermaseptin-PP for Lung Cancer Treatment: In vitro/vivo Evaluation and Anti-tumor Mechanisms Study
Year 2020
Patent
Patent ID CN115637282A
Patent Title Research method of novel antibacterial peptide Dermaeptin-PP
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.