B18KA

General Information


DCTPep ID  DCTPep01015

Peptide Name   B18KA

Sequence  GFQKAKAKALACLAKALA

Sequence Length  18

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MDA-MB-468 Breast adenocarcinoma IC50=135.6±9.3 µM MTT assay 24h 1
SK-BR-3 Breast adenocarcinoma IC50=43.5±11.5 µM MTT assay 24h 1
MCF-7/G11-TR5 Breast cancer IC50=56.4±5.0 µM MTT assay 24h 1
ZR-75-1 Invasive breast carcinoma of no special type IC50=58.9±2.0 µM MTT assay 24h 1
MCF-7 Invasive breast carcinoma of no special type IC50=61.8±3.9 µM MTT assay 24h 1
MCF-7/G11-TR1 Breast cancer IC50=61.8±5.1 µM MTT assay 24h 1
T-47D Invasive breast carcinoma of no special type IC50=78.5±20.9 µM MTT assay 24h 1
MDA-MB-231 Breast adenocarcinoma IC50=83.7±20.1 µM MTT assay 24h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  IC50 on PBMCs was = 92.0 ± 39.6 μM

Target  Not available

Affinity  Not available

Mechanism  may induce cell death through processes involving membrane surface blebs, loss of membrane integrity, PI internalization, LDH release and PS externalization, coupled with coordinated regulation of Src•Erk1/18 signaling and mitochondrial events.



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01015

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C82H143N23O20S1

Absent amino acids  DEHIMNPRSTVWY

Theoretical pI  10.04

Acidic residues  0

Basic residues  4

Polar residues  2

Molecular weight (Average)  1803.24

Molecular weight (Monoisotopic)  1802.06

Common amino acids  A

Net charge  4

Instability index (II)  44.56

Aliphatic index  103.89

Grand average of hydropathicity (GRAVY)  0.544

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 32872253

Title  Development of a Cationic Amphiphilic Helical Peptidomimetic (B18L) As A Novel Anti-Cancer Drug Lead

Doi 10.3390/cancers12092448

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.