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B18KA
General Information
DCTPep ID DCTPep01015
Peptide Name B18KA
Sequence GFQKAKAKALACLAKALA
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MDA-MB-468 | Breast adenocarcinoma | IC50=135.6±9.3 µM | MTT assay | 24h | 1 |
| SK-BR-3 | Breast adenocarcinoma | IC50=43.5±11.5 µM | MTT assay | 24h | 1 |
| MCF-7/G11-TR5 | Breast cancer | IC50=56.4±5.0 µM | MTT assay | 24h | 1 |
| ZR-75-1 | Invasive breast carcinoma of no special type | IC50=58.9±2.0 µM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=61.8±3.9 µM | MTT assay | 24h | 1 |
| MCF-7/G11-TR1 | Breast cancer | IC50=61.8±5.1 µM | MTT assay | 24h | 1 |
| T-47D | Invasive breast carcinoma of no special type | IC50=78.5±20.9 µM | MTT assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | IC50=83.7±20.1 µM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity IC50 on PBMCs was = 92.0 ± 39.6 μM
Target Not available
Affinity Not available
Mechanism may induce cell death through processes involving membrane surface blebs, loss of membrane integrity, PI internalization, LDH release and PS externalization, coupled with coordinated regulation of Src•Erk1/18 signaling and mitochondrial events.
Structure Information
PDB ID Not available
Predicted Structure DCTPep01015
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C82H143N23O20S1
Absent amino acids DEHIMNPRSTVWY
Theoretical pI 10.04
Acidic residues 0
Basic residues 4
Polar residues 2
Molecular weight (Average) 1803.24
Molecular weight (Monoisotopic) 1802.06
Common amino acids A
Net charge 4
Instability index (II) 44.56
Aliphatic index 103.89
Grand average of hydropathicity (GRAVY) 0.544
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 32872253
Title Development of a Cationic Amphiphilic Helical Peptidomimetic (B18L) As A Novel Anti-Cancer Drug Lead
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.