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Cyclic PuroA
General Information
DCTPep ID DCTPep01040
Peptide Name Cyclic PuroA
Sequence FPVTWRWWKWWKG
Sequence Length 13
UniProt ID P33432
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Membrane lysis Binding intracellular targets
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50>250 µg/ml | MTT assay | 24h | 1 |
Hemolytic Activity Sheep erythrocytes: No hemolysis up tp 500 µg/ml
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50>250 µg/ml
Target Not available
Affinity Not available
Mechanism Dual mechanism, with the disruption of the membrane integrity possibly being the main cause of cell death, but only after peptide translocation and binding to intracellular targets.
Structure Information
PDB ID Not available
Predicted Structure DCTPep01040
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond NCB: Phe1<--->Gly13
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C98H123N23O15
Absent amino acids ACDEHILMNQSY
Theoretical pI 11.17
Acidic residues 0
Basic residues 3
Polar residues 2
Molecular weight (Average) 1863.2
Molecular weight (Monoisotopic) 1861.96
Common amino acids W
Net charge 3
Instability index (II) 58.3
Aliphatic index 22.31
Grand average of hydropathicity (GRAVY) -0.962
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 14.760
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33207639
Title Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_16980
This work is licensed under a Creative Commons Attribution 4.0 International License.