Cyclic PuroA

General Information


DCTPep ID  DCTPep01040

Peptide Name   Cyclic PuroA

Sequence  FPVTWRWWKWWKG

Sequence Length  13

UniProt ID  P33432 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

Membrane lysis Binding intracellular targets



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50>250 µg/ml MTT assay 24h 1

Hemolytic Activity  Sheep erythrocytes: No hemolysis up tp 500 µg/ml

Normal (non-cancerous) Cytotoxicity  NIH 3T3: IC50>250 µg/ml

Target  Not available

Affinity  Not available

Mechanism  Dual mechanism, with the disruption of the membrane integrity possibly being the main cause of cell death, but only after peptide translocation and binding to intracellular targets.



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01040

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  NCB: Phe1<--->Gly13

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C98H123N23O15

Absent amino acids  ACDEHILMNQSY

Theoretical pI  11.17

Acidic residues  0

Basic residues  3

Polar residues  2

Molecular weight (Average)  1863.2

Molecular weight (Monoisotopic)  1861.96

Common amino acids  W

Net charge  3

Instability index (II)  58.3

Aliphatic index  22.31

Grand average of hydropathicity (GRAVY)  -0.962

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 27500
  Abs 0.1% (=1 g/l) 14.760

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 33207639

Title  Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells

Doi 10.3390/ijms21228624

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_16980

DCTPep is developed by Dr.Zheng's team.