This work is licensed under a Creative Commons Attribution 4.0 International License.
VS-9
General Information
DCTPep ID DCTPep01050
Peptide Name VS-9
Sequence VKLRSLLCS
Sequence Length 9
UniProt ID Not available
PubChem CID Not available
Origin garlic
Type Native peptide
Classification
ACP Tumor active peptide Cancer targeted peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Molt-4 | Adult T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=0.84 mM | MTT assay | 48h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=1.57 mM | MTT assay | 48h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity PBMC: IC50>2.0 mM
Target groove of the BH3 domain on target proteins
Affinity Not available
Mechanism VS-9 could induce apoptosis and upregulate mRNA levels of caspase 3, caspase 8, caspase 9, and Bax while downregulating Bcl-2, Bcl-xL, and Bcl-w.
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C44H83N13O12S1
Absent amino acids ADEFGHIMNPQTWY
Theoretical pI 9.51
Acidic residues 0
Basic residues 2
Polar residues 3
Molecular weight (Average) 1018.28
Molecular weight (Monoisotopic) 1017.6
Common amino acids L
Net charge 2
Instability index (II) 66.48
Aliphatic index 162.22
Grand average of hydropathicity (GRAVY) 0.900
Half Life
100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33595876
Title A novel peptide isolated from garlic shows anticancer effect against leukemic cell lines via interaction with Bcl-2 family proteins
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.