This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-1RL1
General Information
DCTPep ID DCTPep01055
Peptide Name Brevinin-1RL1
Sequence FFPLIAGLAARFLPKIFCSITKRC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Frog Skin Secretion
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| SW480 | Colon adenocarcinoma | IC50=10.37±0.40 µM | MTS assay | 48h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | IC50=5.44±0.33 µM | MTS assay | 48h | 1 |
| A549 | Lung adenocarcinoma | IC50=5.81±0.23 µM | MTS assay | 48h | 1 |
| HCT 116 | Colon carcinoma | IC50=5.87±0.15 µM | MTS assay | 48h | 1 |
| B16-F10 | Mouse melanoma | IC50=6.65±0.33 µM | MTS assay | 48h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | IC50=6.87±0.51 µM | MTS assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: Moderate Hemolysis
Normal (non-cancerous) Cytotoxicity NCM460: IC50=16.84±0.56 µM; BEAS-2B: IC50=16.57±0.29 µM; HaCaT: IC50=28.67±0.36 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01055
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C130H206N32O27S2
Absent amino acids DEHMNQVWY
Theoretical pI 10.11
Acidic residues 0
Basic residues 4
Polar residues 5
Molecular weight (Average) 2713.38
Molecular weight (Monoisotopic) 2711.52
Common amino acids F
Net charge 4
Instability index (II) 35.68
Aliphatic index 110.00
Grand average of hydropathicity (GRAVY) 1.025
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.046, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33916789
Title Antimicrobial Peptide Brevinin-1RL1 from Frog Skin Secretion Induces Apoptosis and Necrosis of Tumor Cells
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.