This work is licensed under a Creative Commons Attribution 4.0 International License.
Ranatuerin-2
General Information
DCTPep ID DCTPep01058
Peptide Name Ranatuerin-2
Sequence GLFLDTLKGAAKDVAGKLEGLKCKITGCKLP
Sequence Length 31
UniProt ID Not available
PubChem CID Not available
Origin Rana catesbeiana
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| LoVo | Colon adenocarcinoma | IC50=128 µM | MTT assay | 24h | 1 |
| A549 | Lung adenocarcinoma | IC50>128 µM | MTT assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50>128 µM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50>128 µM | MTT assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 0% Hemolysis=20 µg/ml; Sheep erythrocytes: <10% Hemolysis=256 µM
Normal (non-cancerous) Cytotoxicity HEK293T cells: IC50>128 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01058
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys23<--->Cys28
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C143H247N37O40S2
Absent amino acids HMNQRSWY
Theoretical pI 9.19
Acidic residues 3
Basic residues 6
Polar residues 9
Molecular weight (Average) 3188.88
Molecular weight (Monoisotopic) 3186.79
Common amino acids KL
Net charge 3
Instability index (II) -13.61
Aliphatic index 107.10
Grand average of hydropathicity (GRAVY) 0.187
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.039, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 34073203
Title Prediction of Anticancer Peptides with High Efficacy and Low Toxicity by Hybrid Model Based on 3D Structure of Peptides
Year 2021
Literature 2
Pubmed ID 9784389
Title Ranatuerins: antimicrobial peptides isolated from the skin of the American bullfrog, Rana catesbeiana
Year 1998
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.