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EH-APP(1-22)-Melittin (21-26)
General Information
DCTPep ID DCTPep01079
Peptide Name EH-APP(1-22)-Melittin (21-26)
Sequence GEILCNLCTGLINTLENLLTTKRKRQQ
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | LC=50 µM | Trypan blue assay | 1h | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | LC50=8 µM | Trypan blue assay | 1h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=10 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01079
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C130H230N40O41S2
Absent amino acids ADFHMPSVWY
Theoretical pI 8.90
Acidic residues 2
Basic residues 4
Polar residues 11
Molecular weight (Average) 3073.62
Molecular weight (Monoisotopic) 3071.66
Common amino acids L
Net charge 2
Instability index (II) 65.3
Aliphatic index 115.56
Grand average of hydropathicity (GRAVY) -0.300
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.041, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 8146160
Title Cytolytic and antibacterial activity of synthetic peptides derived from amoebapore, the pore-forming peptide of Entamoeba histolytica
Year 1994
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.