Peptide Libraray

CA-ME

General Information

DCTPep ID DCTPep01085

Peptide Name CA-ME

Sequence KWKLFKKIGIGAVLKVLTTG

Sequence Length 20

UniProt ID Not available

PubChem CID 16143280

Origin Ceropin A-Magainin 2

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
NCI-H128	Lung small cell carcinoma; Small cell lung cancer	IC50=28.6 µM	MTT assay	72 h	1
NCI-H146	Lung small cell carcinoma; Small cell lung cancer	IC50=28.6 µM	MTT assay	72 h	1
NCI-H69	Lung small cell carcinoma; Small cell lung cancer	IC50=32.2 µM	MTT assay	72 h	1

Hemolytic Activity Human red blood cells: hemolysis 4.2%(50 μg/mL); hemolysis 14.1%(100 μg/mL)

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep01085

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C107H182N26O23

Absent amino acids CDEHMNPQRSY

Theoretical pI 10.60

Acidic residues 0

Basic residues 5

Polar residues 5

Molecular weight (Average) 2200.78

Molecular weight (Monoisotopic) 2199.39

Common amino acids K

Net charge 5

Instability index (II) -27.73

Aliphatic index 131.50

Grand average of hydropathicity (GRAVY) 0.520

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.499

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 9352466

Title Structure-antitumor and hemolytic activity relationships of synthetic peptides derived from cecropin A-magainin 13 and cecropin A-melittin hybrid peptides

Doi 10.1111/j.1399-3011.1997.tb01469.x

Year 1997

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available