P1

General Information


DCTPep ID  DCTPep01086

Peptide Name   P1

Sequence  KWKLFKKIGIGAVLKVLKKG

Sequence Length  20

UniProt ID  Not available

PubChem CID  Not available

Origin  Ceropin A-Magainin 2

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
NCI-H69 Lung small cell carcinoma; Small cell lung cancer IC50=3.4 µM MTT assay 72 h 1
NCI-H128 Lung small cell carcinoma; Small cell lung cancer IC50=3.5 µM MTT assay 72 h 1
NCI-H146 Lung small cell carcinoma; Small cell lung cancer IC50=4.5 µM MTT assay 72 h 1

Hemolytic Activity  Human red blood cells: hemolysis 0.3%(50 μg/mL); hemolysis 1%(100 μg/mL)

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01086

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C111H192N28O21

Absent amino acids  CDEHMNPQRSTY

Theoretical pI  10.78

Acidic residues  0

Basic residues  7

Polar residues  3

Molecular weight (Average)  2254.92

Molecular weight (Monoisotopic)  2253.48

Common amino acids  K

Net charge  7

Instability index (II)  -31.98

Aliphatic index  131.50

Grand average of hydropathicity (GRAVY)  0.200

Half Life 
  1.3 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  3 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 2.439

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 9352466

Title  Structure-antitumor and hemolytic activity relationships of synthetic peptides derived from cecropin A-magainin 14 and cecropin A-melittin hybrid peptides

Doi 10.1111/j.1399-3011.1997.tb01469.x

Year  1997

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.