Peptide Libraray

General Information

DCTPep ID DCTPep01087

Peptide Name P2

Sequence KWKLFKKIGIGKFLHSATTF

Sequence Length 20

UniProt ID Not available

PubChem CID Not available

Origin Ceropin A-Magainin 2

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
NCI-H69	Lung small cell carcinoma; Small cell lung cancer	IC50=36.2 µM	MTT assay	72 h	1
NCI-H128	Lung small cell carcinoma; Small cell lung cancer	IC50=37.9 µM	MTT assay	72 h	1
NCI-H146	Lung small cell carcinoma; Small cell lung cancer	IC50=47.7 µM	MTT assay	72 h	1

Hemolytic Activity Human red blood cells: hemolysis 0.2%(50 μg/mL); hemolysis 1%(100 μg/mL)

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep01087

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C116H180N28O24

Absent amino acids CDEMNPQRVY

Theoretical pI 10.60

Acidic residues 0

Basic residues 6

Polar residues 5

Molecular weight (Average) 2350.88

Molecular weight (Monoisotopic) 2349.37

Common amino acids K

Net charge 6

Instability index (II) -8.83

Aliphatic index 83.00

Grand average of hydropathicity (GRAVY) 0.010

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.340

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 9352466

Title Structure-antitumor and hemolytic activity relationships of synthetic peptides derived from cecropin A-magainin 15 and cecropin A-melittin hybrid peptides

Doi 10.1111/j.1399-3011.1997.tb01469.x

Year 1997

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available