Peptide Libraray

CMAP-L-25

General Information

DCTPep ID DCTPep01127

Peptide Name CMAP-L-25

Sequence KKFFKKMKKFVKKFF

Sequence Length 15

UniProt ID Not available

PubChem CID Not available

Origin Not available

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MDA-MB-231	Breast adenocarcinoma	At a concentration of 100μM, the 24 h cell viability is approximately 25%(Duncan-test,P<0.01)	MTT assay	24 h	Patent
ZR-75-1	Invasive breast carcinoma of no special type	At a concentration of 100μM, the 24 h cell viability is approximately 41%(Duncan-test,P<0.01)	MTT assay	24 h	Patent
BT-474	Invasive breast carcinoma of no special type	At a concentration of 100μM, the 24 h cell viability is approximately 50%(Duncan-test,P<0.01)	MTT assay	24 h	Patent
MCF-7	Invasive breast carcinoma of no special type	At a concentration of 100μM, the 24 h cell viability is approximately 63%(Duncan-test,P<0.01)	MTT assay	24 h	Patent

Hemolytic Activity Human red blood cells: no hemolytic activity at 12.5 μM, 25 μM, 50 μM, 100 μM

Normal (non-cancerous) Cytotoxicity Less toxic than CDDP on human normal MCF10A and 293FT

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep01127

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C103H161N23O16S1

Absent amino acids ACDEGHILNPQRSTWY

Theoretical pI 10.85

Acidic residues 0

Basic residues 8

Polar residues 0

Molecular weight (Average) 2009.62

Molecular weight (Monoisotopic) 2008.22

Common amino acids K

Net charge 8

Instability index (II) 19.13

Aliphatic index 19.33

Grand average of hydropathicity (GRAVY) -0.740

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID Not available

Title Not available

Doi Not available

Year Not available

Patent

Patent ID CN106831946A

Patent Title Anticancer peptide as well as preparation method and application thereof

Other Iinformation Granted Patent; Family: 2s / 2ex; Family Jurisdictions: CN; Legal Status: Active; Application No: 201611198223; Filed: Dec 22, 2016; Published: Nov 10, 2020; Earliest Priority: Dec 22, 2016; Granted: Nov 10, 2020

Other Published ID CN106831946B