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Pleurocidin, NRC-04
General Information
DCTPep ID DCTPep01211
Peptide Name Pleurocidin, NRC-04
Sequence GWGSFFKKAAHVGKHVGKAALTHYL
Sequence Length 25
PubChem CID 16130552
Origin Pseudopleuronectes americanus
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | 50% Cell death=16 µg/ml | MTT assay | 2 h | 3 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=58.2 ± 3.9 μg/ml | XTT assay | 120 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HEK293: IC50=87.5 ± 11.1 µg/ml; Zebrafish embryos: >10% Killing=5 µg/ml (4, 28, 52hpf embryo)
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01211
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C129H192N36O29
Absent amino acids CDEIMNPQR
Theoretical pI 10.18
Acidic residues 0
Basic residues 7
Polar residues 7
Molecular weight (Average) 2711.17
Molecular weight (Monoisotopic) 2709.47
Common amino acids AGK
Net charge 7
Instability index (II) 4.97
Aliphatic index 70.40
Grand average of hydropathicity (GRAVY) -0.068
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.578
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33247193
Title A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy
Doi Not available
Year 2020
Literature 2
Pubmed ID 18779649
Title Structure-antimicrobial activity relationship between pleurocidin and its enantiomer
Doi Not available
Year 2008
Literature 3
Pubmed ID 21729875
Title The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.