This work is licensed under a Creative Commons Attribution 4.0 International License.
GW-H1
General Information
DCTPep ID DCTPep01213
Peptide Name GW-H1
Sequence GYNYAKKLANLAKKFANALW
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Induce apoptosis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| AGS | Gastric adenocarcinoma | IC50=17 µM | MTT assay | 24 h | 1 |
| J5 [Human hepatocellular carcinoma] | Hepatocellular carcinoma | IC50=20.3 μM | MTT assay | 24 h | Patent |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=243 μM
Target Not available
Affinity Not available
Mechanism GW-H1 treatment induced both apoptosis and autophagy of AGS cell line in the early stage, while caspase-dependent apoptosis was further enhanced by inhibition of autophagy at the final stage.
Structure Information
PDB ID Not available
Predicted Structure DCTPep01213
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C109H166N28O26
Absent amino acids CDEHIMPQRSTV
Theoretical pI 10.00
Acidic residues 0
Basic residues 4
Polar residues 6
Molecular weight (Average) 2284.69
Molecular weight (Monoisotopic) 2283.25
Common amino acids A
Net charge 4
Instability index (II) 24.69
Aliphatic index 83.50
Grand average of hydropathicity (GRAVY) -0.340
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.712
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25380626
Title Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy
Year 2015
Patent
Patent ID TWI448297B
Patent Title A method of using novel synthetic peptides for the preparation of drugs with anti-liver cancer activity
Other Iinformation Granted Patent; Family: 2s / 2ex; Family Jurisdictions: TW; Legal Status: Active; Application No: 99121957; Filed: Jul 5, 2010; Published: Aug 11, 2014; Earliest Priority: Jul 5, 2010; Granted: Aug 11, 2014
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.