Peptide Libraray

General Information

DCTPep ID DCTPep01590

Peptide Name S1

Sequence KKWRKWLAK

Sequence Length 9

UniProt ID Not available

PubChem CID Not available

Origin Cationic antimicrobial peptides (CAMPs)

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
PC-9G	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 80%	MTT assay	3 h	Patent
A549	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 85%	MTT assay	3 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 87%	MTT assay	3 h	Patent
SAS	Tongue squamous cell carcinoma; Squamous cell carcinoma of the oral tongue	When the peptide concentrations was 50μM, the Cell survival rate(%) was 1%	MTT assay	3 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 40%	MTT assay	12 h	Patent
PC-9G	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 50%	MTT assay	12 h	Patent
OECM-1	Gingival squamous cell carcinoma; Squamous cell carcinoma of the oral cavity	When the peptide concentrations was 50μM, the Cell survival rate(%) was 65%	MTT assay	12 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 68%	MTT assay	24 h	Patent
A549	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 72%	MTT assay	12 h	Patent
OECM-1	Gingival squamous cell carcinoma; Squamous cell carcinoma of the oral cavity	When the peptide concentrations was 50μM, the Cell survival rate(%) was 75%	MTT assay	3 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 79%	MTT assay	3 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 79%	MTT assay	12 h	Patent
PC-9G	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 80%	MTT assay	24 h	Patent
SAS	Tongue squamous cell carcinoma; Squamous cell carcinoma of the oral tongue	When the peptide concentrations was 50μM, the Cell survival rate(%) was 81%	MTT assay	12 h	Patent
OECM-1	Gingival squamous cell carcinoma; Squamous cell carcinoma of the oral cavity	When the peptide concentrations was 50μM, the Cell survival rate(%) was 82%	MTT assay	24 h	Patent
SAS	Tongue squamous cell carcinoma; Squamous cell carcinoma of the oral tongue	When the peptide concentrations was 50μM, the Cell survival rate(%) was 82%	MTT assay	24 h	Patent
PC-9	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 97%	MTT assay	24 h	Patent
A549	Lung adenocarcinoma	When the peptide concentrations was 50μM, the Cell survival rate(%) was 98%	MTT assay	24 h	Patent

Hemolytic Activity human red blood cells: no hemolytic activity of the peptide was found even at 800 μM

Normal (non-cancerous) Cytotoxicity human fibroblast: When the peptide concentrations was 100μM, the Cell survival rate(%) was 80%

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Acetylization

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C61H98N18O10

Absent amino acids CDEFGHIMNPQSTVY

Theoretical pI 11.33

Acidic residues 0

Basic residues 5

Polar residues 0

Molecular weight (Average) 1243.56

Molecular weight (Monoisotopic) 1242.77

Common amino acids K

Net charge 5

Instability index (II) 22.6

Aliphatic index 54.44

Grand average of hydropathicity (GRAVY) -1.811

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 8.846

Amino acid distribution

Literature Information

Literature 1

Pubmed ID Not available

Title Not available

Doi Not available

Year Not available

Patent

Patent ID US10093699B2

Patent Title Peptides with antimicrobial, anticancer and/or wound-healing promoting activities, pharmaceutical compositions containing the same, and use of the peptides with antimicrobial, anticancer and/or wound-healing promoting activities

Other Iinformation Granted Patent; Family: 4s / 4ex; Family Jurisdictions: TW, US; Legal Status: Active; Application No: 201615130709; Filed: Apr 15, 2016; Published: Oct 9, 2018; Earliest Priority: Nov 5, 2015; Granted: Oct 9, 2018

Other Published ID TW201716428A TWI619731B US10093699B2