Peptide Libraray

GK-1

General Information

DCTPep ID DCTPep01812

Peptide Name GK-1

Sequence KWKLFKKIGIGRLLKRGLRKLLK

Sequence Length 23

UniProt ID Not available

PubChem CID Not available

Origin AMPs

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
LoVo	Colon adenocarcinoma	IC50=35 μg/ml	MTT assay	Not available	Patent
QGY-7703	Human papillomavirus-related endocervical adenocarcinoma	IC50=38 μg/ml	MTT assay	Not available	Patent
K562	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia	IC50=45 μg/ml	MTT assay	Not available	Patent
BCaP-37	Human papillomavirus-related endocervical adenocarcinoma	IC50=52 μg/ml	MTT assay	Not available	Patent

Hemolytic Activity Human red blood cells: When the concentration of peptide is 12.5, 25, 50, 100, 200 and 500 μg/ml, the hemolysis rate (%) is 0%, 0%, 0.5%, 0.8% and 1.5%, respectively.

Normal (non-cancerous) Cytotoxicity Mouse NH-3T3: IC50>100 μg/ml

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep01812

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C134H238N40O24

Absent amino acids ACDEHMNPQSTVY

Theoretical pI 12.32

Acidic residues 0

Basic residues 10

Polar residues 3

Molecular weight (Average) 2793.62

Molecular weight (Monoisotopic) 2791.86

Common amino acids K

Net charge 10

Instability index (II) -0.26

Aliphatic index 135.65

Grand average of hydropathicity (GRAVY) -0.361

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.969

Amino acid distribution

Literature Information

Literature 1

Pubmed ID Not available

Title Not available

Doi Not available

Year Not available

Patent

Patent ID WO2004/020461A1

Patent Title A Group of Novel Synthetic Antibiotic Peptides

Other Iinformation Patent Application; Family: 10s / 10ex; Family Jurisdictions: US, AU, CN, EP, AT, WO; Legal Status: Pending; Application No: 0300522; Filed: Jul 1, 2003; Published: Mar 11, 2004; Earliest Priority: Sep 2, 2002

Other Published ID AT528316T AU2003293296A1 CN1269837C CN1398897A EP1541584A1 EP1541584A4 EP1541584B1 US2008070279A1 US7629438B2