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GK-3
General Information
DCTPep ID DCTPep01814
Peptide Name GK-3
Sequence RWRLFKRIGIGRLLKRGLR
Sequence Length 19
UniProt ID Not available
PubChem CID Not available
Origin AMPs
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=13 μg/ml | MTT assay | Not available | Patent |
| QGY-7703 | Human papillomavirus-related endocervical adenocarcinoma | IC50=21 μg/ml | MTT assay | Not available | Patent |
| BCaP-37 | Human papillomavirus-related endocervical adenocarcinoma | IC50=25 μg/ml | MTT assay | Not available | Patent |
| LoVo | Colon adenocarcinoma | IC50=31 μg/ml | MTT assay | Not available | Patent |
Hemolytic Activity Human red blood cells: When the concentration of peptide is 12.5, 25, 50, 100, 200 and 500 μg/ml, the hemolysis rate (%) is 0%, 0%, 0.6%, 1.1% and 1.9%, respectively.
Normal (non-cancerous) Cytotoxicity Mouse NH-3T3: IC50>100 μg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01814
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C110H192N40O20
Absent amino acids ACDEHMNPQSTVY
Theoretical pI 12.70
Acidic residues 0
Basic residues 8
Polar residues 3
Molecular weight (Average) 2394.99
Molecular weight (Monoisotopic) 2393.52
Common amino acids R
Net charge 8
Instability index (II) 62.76
Aliphatic index 123.16
Grand average of hydropathicity (GRAVY) -0.521
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.296
Amino acid distribution
Literature Information
Literature 1
Pubmed ID Not available
Title Not available
Doi Not available
Year Not available
Patent
Patent ID WO2004/020461A1
Patent Title A Group of Novel Synthetic Antibiotic Peptides
Other Iinformation Patent Application; Family: 10s / 10ex; Family Jurisdictions: US, AU, CN, EP, AT, WO; Legal Status: Pending; Application No: 0300522; Filed: Jul 1, 2003; Published: Mar 11, 2004; Earliest Priority: Sep 2, 2002
Other Published ID AT528316T AU2003293296A1 CN1269837C CN1398897A EP1541584A1 EP1541584A4 EP1541584B1 US2008070279A1 US7629438B2
This work is licensed under a Creative Commons Attribution 4.0 International License.