GK-3

General Information


DCTPep ID  DCTPep01814

Peptide Name   GK-3

Sequence  RWRLFKRIGIGRLLKRGLR

Sequence Length  19

UniProt ID  Not available

PubChem CID  Not available

Origin  AMPs

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=13 μg/ml MTT assay Not available Patent
QGY-7703 Human papillomavirus-related endocervical adenocarcinoma IC50=21 μg/ml MTT assay Not available Patent
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma IC50=25 μg/ml MTT assay Not available Patent
LoVo Colon adenocarcinoma IC50=31 μg/ml MTT assay Not available Patent

Hemolytic Activity  Human red blood cells: When the concentration of peptide is 12.5, 25, 50, 100, 200 and 500 μg/ml, the hemolysis rate (%) is 0%, 0%, 0.6%, 1.1% and 1.9%, respectively.

Normal (non-cancerous) Cytotoxicity  Mouse NH-3T3: IC50>100 μg/ml

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01814

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C110H192N40O20

Absent amino acids  ACDEHMNPQSTVY

Theoretical pI  12.70

Acidic residues  0

Basic residues  8

Polar residues  3

Molecular weight (Average)  2394.99

Molecular weight (Monoisotopic)  2393.52

Common amino acids  R

Net charge  8

Instability index (II)  62.76

Aliphatic index  123.16

Grand average of hydropathicity (GRAVY)  -0.521

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 2.296

Amino acid distribution



Literature Information


Literature 1

Pubmed ID Not available

Title  Not available

Doi Not available

Year  Not available

Patent

Patent ID WO2004/020461A1

Patent Title  A Group of Novel Synthetic Antibiotic Peptides

Other Iinformation  Patent Application; Family: 10s / 10ex; Family Jurisdictions: US, AU, CN, EP, AT, WO; Legal Status: Pending; Application No: 0300522; Filed: Jul 1, 2003; Published: Mar 11, 2004; Earliest Priority: Sep 2, 2002

Other Published ID  AT528316T  AU2003293296A1  CN1269837C  CN1398897A  EP1541584A1  EP1541584A4  EP1541584B1  US2008070279A1  US7629438B2 




DCTPep is developed by Dr.Zheng's team.