DCTPep ID  DCTPep01974

Peptide Name   B1CTcu5 (Linear Brevinin CTcu5)

Sequence  LIAGLAANFLPQILCKIARKC

Sequence Length  21

UniProt ID  E3SYG2  D2K8D1  C3RTH8 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Hemolytic Activity  Human erythrocytes: 37% Hemolysis=200 µM

Normal (non-cancerous) Cytotoxicity  HEK293: 50%Cytotoxicity>100 µg/ml; PBMC: 50%Cytotoxicity=75.1±1.46 µg/ml

Target  Not available

Affinity  Not available

Mechanism  Not available

PDB ID  Not available

Predicted Structure  DCTPep01974

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L

Grand average of hydropathicity (GRAVY)  1.067

Half Life 
  5.5 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.055, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature 1

Pubmed ID 25997127

Title  Structure-Activity Relationship and Mode of Action of a Frog Secreted Antibacterial Peptide B1CTcu5 Using Synthetically and Modularly Modified or Deleted (SMMD) Peptides

Doi 10.1371/journal.pone.0124210

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available

DBAASP ID  DBAASPS_13651