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B1CTcu5-LIAG
General Information
DCTPep ID DCTPep01975
Peptide Name B1CTcu5-LIAG
Sequence LAANFLPQILCKIARKC
Sequence Length 17
UniProt ID E3SYG2 D2K8D1 C3RTH8
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A431 | Skin squamous cell carcinoma | 50% Cytotoxicity>100 µg/ml | MTT assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 30% Hemolysis=200 µM
Normal (non-cancerous) Cytotoxicity HEK293: 50%Cytotoxicity>100 µg/ml; PBMC: 50%Cytotoxicity>100 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01975
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys11<--->Cys17
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C86H148N24O20S2
Absent amino acids DEGHMSTVWY
Theoretical pI 9.50
Acidic residues 0
Basic residues 3
Polar residues 3
Molecular weight (Average) 1902.39
Molecular weight (Monoisotopic) 1901.07
Common amino acids AL
Net charge 3
Instability index (II) 29.56
Aliphatic index 132.35
Grand average of hydropathicity (GRAVY) 0.747
Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.066, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25997127
Title Structure-Activity Relationship and Mode of Action of a Frog Secreted Antibacterial Peptide B1CTcu5 Using Synthetically and Modularly Modified or Deleted (SMMD) Peptides
Doi 10.1371/journal.pone.0124210
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_13655
This work is licensed under a Creative Commons Attribution 4.0 International License.