B1CTcu5H

General Information


DCTPep ID  DCTPep01979

Peptide Name   B1CTcu5H

Sequence  HIAGLAANFLPQILCKIARKC

Sequence Length  21

UniProt ID  E3SYG2  D2K8D1  C3RTH8 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A431 Skin squamous cell carcinoma 50% Cytotoxicity>100 µg/ml MTT assay Not available 1

Hemolytic Activity  Human erythrocytes: 30.4% Hemolysis=200 µg/ml

Normal (non-cancerous) Cytotoxicity  HEK293: 50%Cytotoxicity>100 µg/ml; PBMC: 50%Cytotoxicity=90±2.56 µg/ml

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01979

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Cys15<--->Cys21

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C103H174N30O24S2

Absent amino acids  DEMSTVWY

Theoretical pI  9.50

Acidic residues  0

Basic residues  4

Polar residues  4

Molecular weight (Average)  2280.82

Molecular weight (Monoisotopic)  2279.28

Common amino acids  A

Net charge  4

Instability index (II)  46.76

Aliphatic index  130.48

Grand average of hydropathicity (GRAVY)  0.733

Half Life 
  3.5 hours (mammalian reticulocytes, in vitro).
  10 min (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.055, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 25997127

Title  Structure-Activity Relationship and Mode of Action of a Frog Secreted Antibacterial Peptide B1CTcu5 Using Synthetically and Modularly Modified or Deleted (SMMD) Peptides

Doi 10.1371/journal.pone.0124210

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_13659

DCTPep is developed by Dr.Zheng's team.