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PTPRJ pep-19.4
General Information
DCTPep ID DCTPep02043
Peptide Name PTPRJ pep-19.4
Sequence CHHALTHAC
Sequence Length 9
UniProt ID Not available
PubChem CID Not available
Origin Synthetic (Derived from PTPRJ pep-19)
Type Synthetic peptide
Classification
ACP Tumor active peptide Cancer targeted peptides PTPRJ agonist
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 48.0 ± 2.82% Inhibition=160 μM | Trypan blue assay | 24 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 62.5 ± 4.9% Inhibition=160 μM | Trypan blue assay | 48 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 66.5 ± 2.12% Inhibition=160 μM | Trypan blue assay | 72 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HUVEC: ~5% Inhibition=160 μM (24 h); ~25% Inhibition=160 μM (48 h); ~35% Inhibition=160 μM (24 h)
Target PTPRJ
Affinity Not available
Mechanism PTPRJ is a receptor-type protein tyrosine phosphatase whose expression is strongly reduced in the majority of investigated cancer cell lines and tumor specimens.It showed tumor suppressor activity in several models.
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys1<--->Cys9
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C40H61N15O11S2
Absent amino acids DEFGIKMNPQRSVWY
Theoretical pI 7.00
Acidic residues 0
Basic residues 3
Polar residues 3
Molecular weight (Average) 992.14
Molecular weight (Monoisotopic) 991.41
Common amino acids H
Net charge 3
Instability index (II) 93.93
Aliphatic index 65.56
Grand average of hydropathicity (GRAVY) 0.233
Half Life
1.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.126, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23627474
Title Discovery of PTPRJ agonist peptides that effectively inhibit in vitro cancer cell proliferation and tube formation
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
This work is licensed under a Creative Commons Attribution 4.0 International License.