Piscidin 3 Cu2+

General Information


DCTPep ID  DCTPep02086

Peptide Name   Piscidin 3 Cu2+

Sequence  FIHHIFRGIVHAGRSIGRFLTG

Sequence Length  22

UniProt ID  Not available

PubChem CID  Not available

Origin  Morone saxatilis (Striped bass) (Perca saxatilis)

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=1.97 µM MTT/MTS assay 24 h, 48 h 1
A549 Lung adenocarcinoma IC50=2.99 µM MTT/MTS assay 24 h, 48 h 1
MDA-MB-231 Breast adenocarcinoma IC50=5.25 µM MTT/MTS assay 24 h, 48 h 1
HT-1080 Fibrosarcoma IC50=5.52 µM MTT/MTS assay 24 h, 48 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Metallating HDPs is an effective strategy to enhance their anticancer properties through the expansion of their mechanism of action, so that both physical and chemical damage of membranes is achieved.



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02086

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Coordination bond: Phe1<---Cu2+--->Phe1 (Amine); Phe1<---Cu2+--->Ile2 (Amide); Phe1<---Cu2+--->His3 (Imidazole)

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C116H179N37O25

Absent amino acids  CDEKMNPQWY

Theoretical pI  12.30

Acidic residues  0

Basic residues  6

Polar residues  6

Molecular weight (Average)  2491.93

Molecular weight (Monoisotopic)  2490.39

Common amino acids  GI

Net charge  6

Instability index (II)  39.66

Aliphatic index  106.36

Grand average of hydropathicity (GRAVY)  0.455

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 34135370

Title  Copper-binding anticancer peptides from the piscidin family: an expanded mechanism that encompasses physical and chemical bilayer disruption

Doi 10.1038/s41598-021-91670-w

Year  2021

Literature 2

Pubmed ID 31354312

Title  Substitution of lysine for isoleucine at the center of the nonpolar face of the antimicrobial peptide, piscidin-1, leads to an increase in the rapidity of bactericidal activity and a reduction in toxicity

Doi 10.2147/IDR.S195872

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.