This work is licensed under a Creative Commons Attribution 4.0 International License.
Alyteserin-2a
General Information
DCTPep ID DCTPep02087
Peptide Name Alyteserin-2a
Sequence ILGKLLSTAAGLLSNL
Sequence Length 16
UniProt ID Not available
PubChem CID Not available
Origin Alytes obstetricans
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MDA-MB-231 | Breast adenocarcinoma | LC50=65 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| A549 | Lung adenocarcinoma | LC50=80 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=80 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HepG2 | Hepatoblastoma | LC50=85 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=140 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02087
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C72H130N18O21
Absent amino acids CDEFHMPQRVWY
Theoretical pI 8.75
Acidic residues 0
Basic residues 1
Polar residues 6
Molecular weight (Average) 1583.93
Molecular weight (Monoisotopic) 1582.97
Common amino acids L
Net charge 1
Instability index (II) 10.8
Aliphatic index 183.12
Grand average of hydropathicity (GRAVY) 1.275
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 22965637
Title Transformation of the naturally occurring frog skin peptide, alyteserin-2a into a potent, non-toxic anti-cancer agent
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_2407
This work is licensed under a Creative Commons Attribution 4.0 International License.