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CRAMP-18
General Information
DCTPep ID DCTPep02181
Peptide Name CRAMP-18
Sequence GEKLKKIGQKIKNFFQKL
Sequence Length 18
UniProt ID P51437
PubChem CID 102602734
Origin Mus musculus (Mouse)
Type Synthetic peptide
Classification
Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | IC50>100 µM | MTT assay | 3 days | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50>100 µM | MTT assay | 3 days | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50>100 µM | MTT assay | 3 days | 1 |
Hemolytic Activity Human erythrocytes: Not active up to 100 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02181
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C101H171N27O24
Absent amino acids ACDHMPRSTVWY
Theoretical pI 10.30
Acidic residues 1
Basic residues 6
Polar residues 3
Molecular weight (Average) 2147.64
Molecular weight (Monoisotopic) 2146.3
Common amino acids K
Net charge 5
Instability index (II) -31.39
Aliphatic index 86.67
Grand average of hydropathicity (GRAVY) -0.889
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 10973820
Title CRAMP analogues having potent antibiotic activity against bacterial, fungal, and tumor cells without hemolytic activity
Year 2000
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_3073
This work is licensed under a Creative Commons Attribution 4.0 International License.