CRAMP-18[Q9K]

General Information


DCTPep ID  DCTPep02183

Peptide Name   CRAMP-18[Q9K]

Sequence  GEKLKKIGKKIKNFFQKL

Sequence Length  18

UniProt ID  P51437 

PubChem CID  Not available

Origin  Synthetic (Derived from CRAMP-18)

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane lysis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
Jurkat Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia IC50=66 µM MTT assay 3 days 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=85 µM MTT assay 3 days 1
A549 Lung adenocarcinoma IC50=88 µM MTT assay 3 days 1

Hemolytic Activity  Human erythrocytes: Not active up to 100 µM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02183

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C102H175N27O23

Absent amino acids  ACDHMPRSTVWY

Theoretical pI  10.40

Acidic residues  1

Basic residues  7

Polar residues  3

Molecular weight (Average)  2147.68

Molecular weight (Monoisotopic)  2146.34

Common amino acids  K

Net charge  6

Instability index (II)  -36.11

Aliphatic index  86.67

Grand average of hydropathicity (GRAVY)  -0.911

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 10973820

Title  CRAMP analogues having potent antibiotic activity against bacterial, fungal, and tumor cells without hemolytic activity

Doi 10.1006/bbrc.2000.3269

Year  2000

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_3075

DCTPep is developed by Dr.Zheng's team.