This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-1BYa
General Information
DCTPep ID DCTPep02290
Peptide Name Brevinin-1BYa
Sequence FLPILASLAAKFGPKLFCLVTKKC
Sequence Length 24
PubChem CID Not available
Origin Rana boylii
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HepG2 | Hepatoblastoma | LC50=6 μM | CytoTox 96 non-radiactive cytotoxicity assay | 1 h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=9 μM | CellTiter-Glo Luminescent Cell Viability assay | 24, 48 h | 1 |
Hemolytic Activity Human erythrocytes: LC50=10 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID 6G4U
Predicted Structure DCTPep02290
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys18<--->Cys24
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C126H206N28O27S2
Absent amino acids DEHMNQRWY
Theoretical pI 9.70
Acidic residues 0
Basic residues 4
Polar residues 5
Molecular weight (Average) 2609.31
Molecular weight (Monoisotopic) 2607.5
Common amino acids L
Net charge 4
Instability index (II) 26.58
Aliphatic index 122.08
Grand average of hydropathicity (GRAVY) 1.075
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.048, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21312033
Title Synthesis, conformational analysis and biological properties of a dicarba derivative of the antimicrobial peptide, brevinin-1BYa
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_3536
This work is licensed under a Creative Commons Attribution 4.0 International License.