Hymenochirin-1B

General Information


DCTPep ID  DCTPep02295

Peptide Name   Hymenochirin-1B

Sequence  IKLSPETKDNLKKVLKGAIKGAIAVAKMV

Sequence Length  29

UniProt ID  W8PRC4  W8NYW9 

PubChem CID  Not available

Origin  Hymenochirus boettgeri

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane lysis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HCT 116 Colon carcinoma IC50=12.76±0.43 μM MTT assay 96 h 2
A549 Lung adenocarcinoma IC50=15.22±0.21 μM MTT assay 96 h 2
HepG2 Hepatoblastoma IC50=8.07±0.21 μM MTT assay 96 h 2
A549 Lung adenocarcinoma LC50=2.5 ± 0.2 μM CellTiter-Glo Luminescent Cell Viability assay 24 h 1
HepG2 Hepatoblastoma LC50=22.5 ± 1.4 μM CellTiter-Glo Luminescent Cell Viability assay 24 h 1
MDA-MB-231 Breast adenocarcinoma LC50=9.0 ± 0.3 μM CellTiter-Glo Luminescent Cell Viability assay 24 h 1
HT-29 Colon adenocarcinoma LC50=9.7 ± 0.2 μM CellTiter-Glo Luminescent Cell Viability assay 24 h 1

Hemolytic Activity  Human erythrocytes: LC50=213 ± 18 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02295

(Please note that there is the predicted structure, predicted by AlphaFold)

SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C139H251N37O37S1

Absent amino acids  CFHQRWY

Theoretical pI  10.13

Acidic residues  2

Basic residues  7

Polar residues  5

Molecular weight (Average)  3064.81

Molecular weight (Monoisotopic)  3062.86

Common amino acids  K

Net charge  5

Instability index (II)  12.77

Aliphatic index  124.48

Grand average of hydropathicity (GRAVY)  0.169

Half Life 
  20 hours (mammalian reticulocytes, in vitro).
  30 min (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 24185042

Title  Anti-tumor activities of the host-defense peptide hymenochirin-1B

Doi 10.1016/j.regpep.2013.10.006

Year  2013

Literature 2

Pubmed ID 30789695

Title  Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy

Doi 10.1021/acschembio.9b00046

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_3785

DCTPep is developed by Dr.Zheng's team.