This work is licensed under a Creative Commons Attribution 4.0 International License.
Hymenochirin-1B
General Information
DCTPep ID DCTPep02295
Peptide Name Hymenochirin-1B
Sequence IKLSPETKDNLKKVLKGAIKGAIAVAKMV
Sequence Length 29
PubChem CID Not available
Origin Hymenochirus boettgeri
Type Native peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | IC50=12.76±0.43 μM | MTT assay | 96 h | 2 |
| A549 | Lung adenocarcinoma | IC50=15.22±0.21 μM | MTT assay | 96 h | 2 |
| HepG2 | Hepatoblastoma | IC50=8.07±0.21 μM | MTT assay | 96 h | 2 |
| A549 | Lung adenocarcinoma | LC50=2.5 ± 0.2 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HepG2 | Hepatoblastoma | LC50=22.5 ± 1.4 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=9.0 ± 0.3 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=9.7 ± 0.2 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: LC50=213 ± 18 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02295
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C139H251N37O37S1
Absent amino acids CFHQRWY
Theoretical pI 10.13
Acidic residues 2
Basic residues 7
Polar residues 5
Molecular weight (Average) 3064.81
Molecular weight (Monoisotopic) 3062.86
Common amino acids K
Net charge 5
Instability index (II) 12.77
Aliphatic index 124.48
Grand average of hydropathicity (GRAVY) 0.169
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24185042
Title Anti-tumor activities of the host-defense peptide hymenochirin-1B
Doi 10.1016/j.regpep.2013.10.006
Year 2013
Literature 2
Pubmed ID 30789695
Title Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy
Doi 10.1021/acschembio.9b00046
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_3785
This work is licensed under a Creative Commons Attribution 4.0 International License.