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Hymenochirin-4B
General Information
DCTPep ID DCTPep02298
Peptide Name Hymenochirin-4B
Sequence IKIPAFVKDTLKKVAKGVISAVAGALTQ
Sequence Length 28
UniProt ID W8NYW4
PubChem CID Not available
Origin Hymenochirus boettgeri
Type Native peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MDA-MB-231 | Breast adenocarcinoma | LC50=11.0 ± 1.7 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=14.8 ± 2.0 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| HepG2 | Hepatoblastoma | LC50=28.4 ± 0.4 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
| A549 | Lung adenocarcinoma | LC50=5.9 ± 0.6 μM | CellTiter-Glo Luminescent Cell Viability assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: LC50=158 ± 6 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02298
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C133H232N34O35
Absent amino acids CEHMNRWY
Theoretical pI 10.18
Acidic residues 1
Basic residues 5
Polar residues 5
Molecular weight (Average) 2867.51
Molecular weight (Monoisotopic) 2865.74
Common amino acids AK
Net charge 4
Instability index (II) -11.79
Aliphatic index 128.93
Grand average of hydropathicity (GRAVY) 0.664
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24185042
Title Anti-tumor activities of the host-defense peptide hymenochirin-1B
Doi 10.1016/j.regpep.2013.10.006
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_3788
This work is licensed under a Creative Commons Attribution 4.0 International License.