Peptide Libraray

Hymenochirin-1B [D9K]

General Information

DCTPep ID DCTPep02301

Peptide Name Hymenochirin-1B [D9K]

Sequence IKLSPETKKNLKKVLKGAIKGAIAVAKMV

Sequence Length 29

UniProt ID Not available

PubChem CID Not available

Origin Synthetic (Derived from Hymenochirin-1B)

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane lysis

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
A549	Lung adenocarcinoma	IC50=1.82±0.23 μM	MTT assay	96 h	2
HepG2	Hepatoblastoma	IC50=4.96±0.43 μM	MTT assay	96 h	2
HCT 116	Colon carcinoma	IC50=6.50±0.32 μM	MTT assay	96 h	2
A549	Lung adenocarcinoma	LC50=1.1 ± 0.1 μM	CellTiter-Glo Luminescent Cell Viability assay	24 h	1
MDA-MB-231	Breast adenocarcinoma	LC50=2.9 ± 0.5 μM	CellTiter-Glo Luminescent Cell Viability assay	24 h	1
HepG2	Hepatoblastoma	LC50=3.9 ± 0.1 μM	CellTiter-Glo Luminescent Cell Viability assay	24 h	1
HT-29	Colon adenocarcinoma	LC50=6.5 ± 1.2 μM	CellTiter-Glo Luminescent Cell Viability assay	24 h	1

Hemolytic Activity Human erythrocytes: LC50=174 ± 12 μM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02301

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C141H258N38O35S1

Absent amino acids CDFHQRWY

Theoretical pI 10.48

Acidic residues 1

Basic residues 8

Polar residues 5

Molecular weight (Average) 3077.89

Molecular weight (Monoisotopic) 3075.93

Common amino acids K

Net charge 7

Instability index (II) 12.77

Aliphatic index 124.48

Grand average of hydropathicity (GRAVY) 0.155

Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 24185042

Title Anti-tumor activities of the host-defense peptide hymenochirin-1B

Doi 10.1016/j.regpep.2013.10.006

Year 2013

Literature 2

Pubmed ID 30789695

Title Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy

Doi 10.1021/acschembio.9b00046

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPS_4422