This work is licensed under a Creative Commons Attribution 4.0 International License.
m2163
General Information
DCTPep ID DCTPep02325
Peptide Name m2163
Sequence KRKCPKTPFDNTPGAWFAHLILGC
Sequence Length 24
UniProt ID Q036G0 K0N7G0 K6RXD6
PubChem CID Not available
Origin Lactobacillus casei; Lactobacillus paracasei
Type Native peptide
Classification
ACP Tumor active peptide Induce apoptosis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| BFTC-905 | Bladder carcinoma | ~40% Cell death=40 µg/ml | MTT assay | 24 h | 1 |
| CaCo-2 | Colon adenocarcinoma | ~40% Cell death=40 µg/ml | MTT assay | 24 h | 1 |
| SW480 | Colon adenocarcinoma | IC50=40 µg/ml | MTT assay | 24 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity H184B5F5/M10: ~20% Cell death=40 µg/ml; CHO-K1: ~40% Cell death=40 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02325
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys4<--->Cys24
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C124H190N34O30S2
Absent amino acids EMQSVY
Theoretical pI 9.39
Acidic residues 1
Basic residues 5
Polar residues 7
Molecular weight (Average) 2701.2
Molecular weight (Monoisotopic) 2699.38
Common amino acids KP
Net charge 4
Instability index (II) 41.77
Aliphatic index 57.08
Grand average of hydropathicity (GRAVY) -0.333
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 2.082, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.036, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25557887
Title Antimicrobial peptide m2163 or m2386 identified from Lactobacillus casei ATCC 334 can trigger apoptosis in the human colorectal cancer cell line SW480
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4143
This work is licensed under a Creative Commons Attribution 4.0 International License.