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m2386
General Information
DCTPep ID DCTPep02326
Peptide Name m2386
Sequence DSIRDVSPTFNKIRRWFDGLFK
Sequence Length 22
PubChem CID Not available
Origin Lactobacillus casei; Lactobacillus paracasei
Type Native peptide
Classification
ACP Tumor active peptide Induce apoptosis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| BFTC-905 | Bladder carcinoma | ~40% Cell death=40 µg/ml | MTT assay | 24 h | 1 |
| CaCo-3 | Colon adenocarcinoma | ~40% Cell death=40 µg/ml | MTT assay | 24 h | 1 |
| SW480 | Colon adenocarcinoma | IC50=40 µg/ml | MTT assay | 24 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity H184B5F5/M10: ~20% Cell death=40 µg/ml; CHO-K1: ~35% Cell death=40 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02326
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C124H189N35O33
Absent amino acids ACEHMQY
Theoretical pI 9.98
Acidic residues 3
Basic residues 5
Polar residues 5
Molecular weight (Average) 2698.08
Molecular weight (Monoisotopic) 2696.42
Common amino acids DFR
Net charge 2
Instability index (II) 101.64
Aliphatic index 66.36
Grand average of hydropathicity (GRAVY) -0.686
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.038
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25557887
Title Antimicrobial peptide m2163 or m2386 identified from Lactobacillus casei ATCC 334 can trigger apoptosis in the human colorectal cancer cell line SW480
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4142
This work is licensed under a Creative Commons Attribution 4.0 International License.