Peptide Libraray

Palustrin-2AJ1

General Information

DCTPep ID DCTPep02330

Peptide Name Palustrin-2AJ1

Sequence GFMDTAKNVAKNVAVTLIDKLRCKVTGGC

Sequence Length 29

UniProt ID G3ETQ3 G3F829 G3F830

PubChem CID Not available

Origin Amolops jingdongensis

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HT-29	Colon adenocarcinoma	IC50=57 µM	MTT assay	48 h	1
K562	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia	IC50=58 µM	MTT assay	48 h	1

Hemolytic Activity Human erythrocytes: 50% Hemolysis=150 µM; Rabbit erythrocytes: 50% Hemolysis=150 µM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02330

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys23<--->Cys29

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C131H226N38O39S3

Absent amino acids EHPQSWY

Theoretical pI 9.31

Acidic residues 2

Basic residues 5

Polar residues 10

Molecular weight (Average) 3053.65

Molecular weight (Monoisotopic) 3051.6

Common amino acids KV

Net charge 3

Instability index (II) -2.2

Aliphatic index 90.69

Grand average of hydropathicity (GRAVY) 0.228

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.041, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 21816202

Title Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis

Doi 10.1016/j.biochi.2011.07.021

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPR_4195