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Palustrin-2ISb (1-29), GL-29
General Information
DCTPep ID DCTPep02359
Peptide Name Palustrin-2ISb (1-29), GL-29
Sequence GLWNSIKIAGKKLFVNVLDKIRCKVAGGC
Sequence Length 29
UniProt ID G5ELM2
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| H157 | Buccal mucosa squamous cell carcinoma; Squamous cell carcinoma of the oral cavity | IC50=1.74 μM | MTT assay | 24 h | 1 |
| HCT 116 | Colon carcinoma | IC50=17.86 μM | MTT assay | 24 h | 1 |
| NCI-H838 | Lung adenocarcinoma | IC50=5.38 μM | MTT assay | 24 h | 1 |
| U-251MG | Astrocytoma | IC50=6.6 μM | MTT assay | 24 h | 1 |
Hemolytic Activity Horse erythrocytes: 10% Hemolysis=5.86 µM; 50% Hemolysis=33.03 µM
Normal (non-cancerous) Cytotoxicity Human keratinocytes HaCat: IC50=5.05 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02359
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys23<--->Cys29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C142H240N40O35S2
Absent amino acids EHMPQTY
Theoretical pI 9.90
Acidic residues 1
Basic residues 6
Polar residues 9
Molecular weight (Average) 3131.83
Molecular weight (Monoisotopic) 3129.77
Common amino acids K
Net charge 5
Instability index (II) 5.84
Aliphatic index 117.59
Grand average of hydropathicity (GRAVY) 0.383
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 1.796, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.756, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 36009917
Title Targeted Modification and Structure-Activity Study of GL-29, an Analogue of the Antimicrobial Peptide Palustrin-2ISb
Doi 10.3390/antibiotics11081048
Year 2022
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_4313
This work is licensed under a Creative Commons Attribution 4.0 International License.