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GL-22
General Information
DCTPep ID DCTPep02360
Peptide Name GL-22
Sequence GLWNSIKIAGKKLFVNVLDKIR
Sequence Length 22
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | IC50=128.89 μM | MTT assay | 24 h | 1 |
| NCI-H838 | Lung adenocarcinoma | IC50=25.25 μM | MTT assay | 24 h | 1 |
| U-251MG | Astrocytoma | IC50=40.58 μM | MTT assay | 24 h | 1 |
| H157 | Buccal mucosa squamous cell carcinoma; Squamous cell carcinoma of the oral cavity | IC50=9.36 μM | MTT assay | 24 h | 1 |
Hemolytic Activity Horse erythrocytes: 10% Hemolysis=87.304 µM; 50% Hemolysis=254.11 µM
Normal (non-cancerous) Cytotoxicity Human keratinocytes HaCat: IC50=38.90 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02360
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C118H198N32O28
Absent amino acids CEHMPQTY
Theoretical pI 10.46
Acidic residues 1
Basic residues 5
Polar residues 5
Molecular weight (Average) 2513.07
Molecular weight (Monoisotopic) 2511.51
Common amino acids K
Net charge 4
Instability index (II) 2.76
Aliphatic index 137.27
Grand average of hydropathicity (GRAVY) 0.218
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.189
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 36009917
Title Targeted Modification and Structure-Activity Study of GL-29, an Analogue of the Antimicrobial Peptide Palustrin-2ISb
Doi 10.3390/antibiotics11081048
Year 2022
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.