This work is licensed under a Creative Commons Attribution 4.0 International License.
Pentadactylin
General Information
DCTPep ID DCTPep02364
Peptide Name Pentadactylin
Sequence GLLDTLKGAAKNVVGSLASKVMEKL
Sequence Length 25
UniProt ID P0DQJ7
PubChem CID Not available
Origin Leptodactylus pentadactylus
Type Native peptide
Classification
ACP Tumor active peptide Induce apoptosis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| B16-F10 | Mouse melanoma | IC50=25.7 µM | MTT assay | Not available | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Human fibroblasts FHN: IC50=35.9 µM
Target Not available
Affinity Not available
Mechanism In the present study the rounding and cell shrinkage, DNA fragmentation, mitochondrial membrane potential alteration and S phase arrest support the apoptogenic nature of pentadactylin, whereas cell growth inhibition and reduction of cell viability indicate its anti-proliferative and cytotoxic activity.
Structure Information
PDB ID Not available
Predicted Structure DCTPep02364
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C112H200N30O34S1
Absent amino acids CFHIPQRWY
Theoretical pI 9.53
Acidic residues 2
Basic residues 4
Polar residues 7
Molecular weight (Average) 2543.06
Molecular weight (Monoisotopic) 2541.46
Common amino acids L
Net charge 2
Instability index (II) -16.79
Aliphatic index 124.80
Grand average of hydropathicity (GRAVY) 0.372
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20012549
Title Anti-proliferative and cytotoxic activity of pentadactylin isolated from Leptodactylus labyrinthicus on melanoma cells
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4394
This work is licensed under a Creative Commons Attribution 4.0 International License.