Peptide Libraray

Temporin-1CEa

General Information

DCTPep ID DCTPep02396

Peptide Name Temporin-1CEa

Sequence FVDLKKIANIINSIFGK

Sequence Length 17

UniProt ID B8QP36

PubChem CID Not available

Origin Rana chensinensis

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MCF-7	Invasive breast carcinoma of no special type	IC50=31.48 µM	MTT assay	24 h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	IC50=36.31 µM	MTT assay	24 h	2
BCaP-37	Human papillomavirus-related endocervical adenocarcinoma	IC50=37.52 µM	MTT assay	24 h	1
BEL-7402	Human papillomavirus-related endocervical adenocarcinoma	IC50=38.98 µM	MTT assay	24 h	2
SMMC-7721	Human papillomavirus-related endocervical adenocarcinoma	IC50=50.32 µM	MTT assay	24 h	2
A549	Lung adenocarcinoma	IC50=53.31 µM	MTT assay	24 h	2
BGC-823	Human papillomavirus-related endocervical adenocarcinoma	IC50=61.63 µM	MTT assay	24 h	2
LK-2	Lung squamous cell carcinoma	IC50=63.4 µM	MTT assay	24 h	2
HO-8910	Human papillomavirus-related endocervical adenocarcinoma	IC50=64.75 µM	MTT assay	24 h	2
MDA-MB-231	Breast adenocarcinoma	IC50=65.42 µM	MTT assay	24 h	1
NCI-H446	Lung small cell carcinoma; Small cell lung cancer	IC50=67.66 µM	MTT assay	24 h	2
HT-29	Colon adenocarcinoma	IC50>100 µM	MTT assay	24 h	2

Hemolytic Activity Human erythrocytes: LC50=95.74 µM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02396

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C91H150N22O23

Absent amino acids CEHMPQRTWY

Theoretical pI 9.70

Acidic residues 1

Basic residues 3

Polar residues 4

Molecular weight (Average) 1920.33

Molecular weight (Monoisotopic) 1919.12

Common amino acids I

Net charge 2

Instability index (II) 11.44

Aliphatic index 137.65

Grand average of hydropathicity (GRAVY) 0.588

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 23609760

Title Design of potent, non-toxic anticancer peptides based on the structure of the antimicrobial peptide, temporin-1CEa

Doi 10.1007/s12272-013-0112-8

Year 2013

Literature 2

Pubmed ID 21871946

Title Antitumor effects and cell selectivity of temporin-1CEa, an antimicrobial peptide from the skin secretions of the Chinese brown frog (Rana chensinensis)

Doi 10.1016/j.biochi.2011.08.011

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPR_4544