Peptide Libraray

Ascaphin-8

General Information

DCTPep ID DCTPep02465

Peptide Name Ascaphin-8

Sequence GFKDLLKGAAKALVKTVLF

Sequence Length 19

UniProt ID P0CJ32

PubChem CID 16143925

Origin Ascaphus truei

Type Native peptide

Classification

ACP Tumor active peptide Membrane lysis

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HepG2	Hepatoblastoma	LC50=20 µM	CytoTox 96 non-radiactive cytotoxicity assay	1 h	1

Hemolytic Activity Human erythrocytes: LC50=55 µM

Normal (non-cancerous) Cytotoxicity L929 fibroblast-derived cells: LC50=7 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02465

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C97H163N23O23

Absent amino acids CEHIMNPQRSWY

Theoretical pI 10.00

Acidic residues 1

Basic residues 4

Polar residues 3

Molecular weight (Average) 2019.5

Molecular weight (Monoisotopic) 2018.23

Common amino acids KL

Net charge 3

Instability index (II) -13.36

Aliphatic index 128.42

Grand average of hydropathicity (GRAVY) 0.737

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 18554256

Title Design of potent, non-toxic antimicrobial agents based upon the naturally occurring frog skin peptides, ascaphin-8 and peptide XT-7

Doi 10.1111/j.1747-0285.2008.00671.x

Year 2008

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPR_2300