[Lys14] Ascaphin-8

General Information


DCTPep ID  DCTPep02469

Peptide Name   [Lys14] Ascaphin-8

Sequence  GFKDLLKGAAKALKKTVLF

Sequence Length  19

UniProt ID  P0CJ32 

PubChem CID  Not available

Origin  Synthetic (Derived from Ascaphin-8)

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane lysis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HepG2 Hepatoblastoma LC50=200 µM CytoTox 96 non-radiactive cytotoxicity assay  1 h 1

Hemolytic Activity  Human erythrocytes: LC50>500 µM

Normal (non-cancerous) Cytotoxicity  L929 fibroblast-derived cells: LC50=110 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02469

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C98H166N24O23

Absent amino acids  CEHIMNPQRSWY

Theoretical pI  10.18

Acidic residues  1

Basic residues  5

Polar residues  3

Molecular weight (Average)  2048.54

Molecular weight (Monoisotopic)  2047.26

Common amino acids  K

Net charge  4

Instability index (II)  -16.31

Aliphatic index  113.16

Grand average of hydropathicity (GRAVY)  0.311

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 18554256

Title  Design of potent, non-toxic antimicrobial agents based upon the naturally occurring frog skin peptides, ascaphin-8 and peptide XT-7

Doi 10.1111/j.1747-0285.2008.00671.x

Year  2008

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_4908

DCTPep is developed by Dr.Zheng's team.