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[Ala16] Ascaphin-8
General Information
DCTPep ID DCTPep02476
Peptide Name [Ala16] Ascaphin-8
Sequence GFKDLLKGAAKALVKAVLF
Sequence Length 19
UniProt ID P0CJ32
PubChem CID Not available
Origin Synthetic (Derived from Ascaphin-8)
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HepG2 | Hepatoblastoma | LC50=9 µM | CytoTox 96 non-radiactive cytotoxicity assay | 1 h | 1 |
Hemolytic Activity Human erythrocytes: LC50=22 µM
Normal (non-cancerous) Cytotoxicity L929 fibroblast-derived cells: LC50=5 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02476
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C96H161N23O22
Absent amino acids CEHIMNPQRSTWY
Theoretical pI 10.00
Acidic residues 1
Basic residues 4
Polar residues 2
Molecular weight (Average) 1989.48
Molecular weight (Monoisotopic) 1988.22
Common amino acids AKL
Net charge 3
Instability index (II) -13.36
Aliphatic index 133.68
Grand average of hydropathicity (GRAVY) 0.868
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 18554256
Title Design of potent, non-toxic antimicrobial agents based upon the naturally occurring frog skin peptides, ascaphin-8 and peptide XT-7
Doi 10.1111/j.1747-0285.2008.00671.x
Year 2008
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_4918
This work is licensed under a Creative Commons Attribution 4.0 International License.