Kalata B1 [N15Y]

General Information


DCTPep ID  DCTPep02585

Peptide Name   Kalata B1 [N15Y]

Sequence  GLPVCGETCVGGTCYTPGCTCSWPVCTRN

Sequence Length  29

UniProt ID  P56254 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane lysis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MM96L Melanoma CC50=12.3±0.6 μM Resazurin assay 2, 24 h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia CC50=3.2±0.1 μM Resazurin assay 2, 24 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma CC50=8.1±0.4 μM Resazurin assay 2, 24 h 1

Hemolytic Activity  Human erythrocytes: 50% Hemolysis=6.9±0.3 µM

Normal (non-cancerous) Cytotoxicity  HFF-1: CC50=6.7±0.6 µM; PBMCs: CC50=1.0±0.1 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02585

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C122H190N34O40S6

Absent amino acids  ADFHIKMQ

Theoretical pI  5.96

Acidic residues  1

Basic residues  1

Polar residues  19

Molecular weight (Average)  2965.41

Molecular weight (Monoisotopic)  2963.22

Common amino acids  C

Net charge  0

Instability index (II)  46.59

Aliphatic index  43.45

Grand average of hydropathicity (GRAVY)  0.228

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 7365
  Abs 0.1% (=1 g/l) 2.484, assuming all pairs of Cys residues form cystines
  Ext. coefficient 6990
  Abs 0.1% (=1 g/l) 2.357, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 25099014

Title  Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting

Doi 10.1002/cbic.201402144

Year  2014

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_12200

DCTPep is developed by Dr.Zheng's team.