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Kalata B1 [N15Y]
General Information
DCTPep ID DCTPep02585
Peptide Name Kalata B1 [N15Y]
Sequence GLPVCGETCVGGTCYTPGCTCSWPVCTRN
Sequence Length 29
UniProt ID P56254
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MM96L | Melanoma | CC50=12.3±0.6 μM | Resazurin assay | 2, 24 h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=3.2±0.1 μM | Resazurin assay | 2, 24 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=8.1±0.4 μM | Resazurin assay | 2, 24 h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=6.9±0.3 µM
Normal (non-cancerous) Cytotoxicity HFF-1: CC50=6.7±0.6 µM; PBMCs: CC50=1.0±0.1 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02585
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C122H190N34O40S6
Absent amino acids ADFHIKMQ
Theoretical pI 5.96
Acidic residues 1
Basic residues 1
Polar residues 19
Molecular weight (Average) 2965.41
Molecular weight (Monoisotopic) 2963.22
Common amino acids C
Net charge 0
Instability index (II) 46.59
Aliphatic index 43.45
Grand average of hydropathicity (GRAVY) 0.228
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 7365
Abs 0.1% (=1 g/l) 2.484, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.357, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25099014
Title Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_12200
This work is licensed under a Creative Commons Attribution 4.0 International License.