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Kalata B1 [N15D]
General Information
DCTPep ID DCTPep02586
Peptide Name Kalata B1 [N15D]
Sequence GLPVCGETCVGGTCDTPGCTCSWPVCTRN
Sequence Length 29
UniProt ID P56254
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Not active up to 60 µM | Resazurin assay | 2, 24 h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Not active up to 60 µM | Resazurin assay | 2, 24 h | 1 |
| MM96L | Melanoma | Not active up to 60 µM | Resazurin assay | 2, 24 h | 1 |
Hemolytic Activity Human erythrocytes: Not active up to 50 µM
Normal (non-cancerous) Cytotoxicity HFF-1: Not active up to 60 µM; PBMCs: Not active up to 60 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02586
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C117H186N34O41S6
Absent amino acids AFHIKMQY
Theoretical pI 4.37
Acidic residues 2
Basic residues 1
Polar residues 18
Molecular weight (Average) 2917.33
Molecular weight (Monoisotopic) 2915.18
Common amino acids C
Net charge -1
Instability index (II) 50.98
Aliphatic index 43.45
Grand average of hydropathicity (GRAVY) 0.152
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5875
Abs 0.1% (=1 g/l) 2.014, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.885, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25099014
Title Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_12201
This work is licensed under a Creative Commons Attribution 4.0 International License.