This work is licensed under a Creative Commons Attribution 4.0 International License.
Kalata B1 [T16S]
General Information
DCTPep ID DCTPep02588
Peptide Name Kalata B1 [T16S]
Sequence GLPVCGETCVGGTCNSPGCTCSWPVCTRN
Sequence Length 29
UniProt ID P56254
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=43.8±0.5 μM | Resazurin assay | 2, 24 h | 1 |
Hemolytic Activity Human erythrocytes: Weak active up to 50 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02588
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C116H185N35O40S6
Absent amino acids ADFHIKMQY
Theoretical pI 5.96
Acidic residues 1
Basic residues 1
Polar residues 19
Molecular weight (Average) 2902.32
Molecular weight (Monoisotopic) 2900.18
Common amino acids C
Net charge 0
Instability index (II) 64.67
Aliphatic index 43.45
Grand average of hydropathicity (GRAVY) 0.148
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5875
Abs 0.1% (=1 g/l) 2.024, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.895, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25099014
Title Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_12208
This work is licensed under a Creative Commons Attribution 4.0 International License.